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1.50658

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonym(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
3 μm
Matrix active group:
ZIC-cHILIC bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle
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Product Name

SeQuant® ZIC®-cHILIC 3 µm, 100 Å 150 x 2.1 mm, suitable for HPLC, PEEK-lined stainless steel column

material

PEEK-lined stainless steel column

Quality Level

100

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure, 70 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

15 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-cHILIC bonding phase

particle size

3 μm

pore size

100 Å pore size

operating pH

1.5-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

room temp

General description

SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Benefits of SPP Zwitterionic HILIC Column

Comparative analysis of SPP Ascentis® Express ZIC®-cHILIC and FPP SeQuant® ZIC®-cHILIC shows that the SPP-based column delivers faster HILIC separations.

HILIC Stationary Phase for Glycan Profiling

Glycan profiling of monoclonal antibodies using Ascentis® Express ZIC®-cHILIC columns enables high-resolution IgG glycan separation with improved efficiency and speed.