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Merck

1.51913

Supelco

Purospher® STAR NH2 (5 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC Column

Synonym(s):

Purospher® STAR NH2 (5µm) Hibar® RT 250-4.6

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Product Name

Purospher® STAR NH2 (5 µm), L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column

material

stainless steel column

Agency

suitable for USP L8

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

extent of labeling

3.5% Carbon loading

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

25 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

NH2 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

Related Categories

General description

Purospher® STAR NH2, 5 µm HPLC columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water. In terms of their polarity, these columns lie between bare silica and reversed-phase silica. Hence, Purospher® STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3 +X-) and therefore display the characteristics of a weak anion exchanger. Medium polarity Purospher® STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed-phase (HILIC) and normal-phase conditions. However, retention is weaker than on silica or RP-supports. Purospher® STAR NH2 columns are based on ultra-pure fully porous silica particles (Type B). Purospher® STAR NH2, 5 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The recommended guard column is Purospher® STAR NH2 (5 µm) LiChroCART™ 4-4 part number 1.50267.0001 [https://www.sigmaaldrich.com/US/en/product/mm/150267]. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART™ cartridges, part number 1.51487.0001 can be used. The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for the HPLC separation of complex.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended for the separation of alcohols, carboxylic acids, fat-soluble vitamins, nucleotides, steroids, sugars, sugar alcohols, sweeteners, and water-soluble vitamins.

Features and Benefits

  • Reproducible results from run-to-run and batch-to-batch

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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