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16-305

Sigma-Aldrich

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor™ 488

clone 11E6, Upstate®, from mouse

Synonym(s):

PKB, PKD-alpha, AKT1, Protein Kinase B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

11E6, monoclonal

species reactivity

rat, canine, human, mouse

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001014431.1
NM_001014432.1
NM_005163.2

UniProt accession no.

P31749

shipped in

wet ice

target post-translational modification

phosphorylation (pSer473)

Related Categories

General description

60 kDa

Immunogen

KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.

Application

Detect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

Recognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.

Physical form

Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

1 year at 4°C from date of shipment

Analysis Note

Routinely evaluated by immunocytochemistry and flow cytometry.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
16-30504053252510816

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