Skip to Content
Merck

17-10085

Magna ChIP® A/G Chromatin Immunoprecipitation Kit

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A/G beads.

Synonym(s):

Magnetic ChIP Kit, Magnetic Chromatin Immunoprecipitation

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(3)

About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.52

Quality Level

100

manufacturer/tradename

Magna ChIP®

technique(s)

immunoprecipitation (IP): suitable

shipped in

dry ice

Related Categories

General description

Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
Unlike standard ChIP protocols that can be laborious and time consuming, the Magna ChIP kit protocol can reduce the amount of time required to perform a ChIP experiment from three days to one. Additionally, the smaller Magna ChIP reaction volume increases the relative concentration of the antibody enabling the ChIP reaction to be performed with reduced amounts of both antibody and sheared chromatin. Finally because this kit uses a blend of protein A and protein G beads, a wider range of antibody isotypes can be used than A or G alone. This allows a wider variety of antibodies to be used and avoids the need to purchase separate kits for protein A and protein G based immunoprecipitation. Because Magna ChIP kits use paramagnetic beads they are compatible with automated high throughput platforms, thus allowing a large number of ChIP reactions to be carried out simultaneously. Features & Benefits:
  • Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day
  • Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone
  • Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions
  • Complete kit with all required reagents for reliable and reproducible results
  • Compatible with ChIPAb+ validated antibody and primer sets

Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Application

Research Category
Epigenetics & Nuclear Function
Used to detect/quantify: Protein A+G

Packaging

Kit capacity: 22 chromatin immunoprecipitation assays

Physical form

Two boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Preparation Note

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Other Notes

Magnetic Protein A/G Beads

ChIP Dilution Buffer

Low Salt Wash Buffer

High Salt Wash Buffer

LiCl Wash Buffer

TE Buffer

Cell Lysis Buffer

Nuclear Lysis Buffer

ChIP Elution Buffer (w/o Proteinase K)

Proteinase K

RNase A

10X Glycine

10X PBS

Protease Inhibitor Cocktail II

Spin Filters

Collection Tubes

Bind Reagent A

Wash Reagent B

Elution Reagent C

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Human ?-defensin expression is not dependent on CCAAT/enhancer binding protein-? in a murine model.
Glenth?j, A; Dahl, S; Larsen, MT; Cowland, JB; Borregaard, N
Testing null
The cross-talk between canonical and non-canonical Wnt-dependent pathways regulates P-glycoprotein expression in human blood-brain barrier cells.
Pinzon-Daza, ML; Salaroglio, IC; Kopecka, J; Garzon, R; Couraud, PO; Ghigo, D; Riganti, C
Journal of Cerebral Blood Flow and Metabolism null
CREB regulates the expression of neuronal glucose transporter 3: a possible mechanism related to impaired brain glucose uptake in Alzheimer's disease.
Jin, N; Qian, W; Yin, X; Zhang, L; Iqbal, K; Grundke-Iqbal, I; Gong, CX; Liu, F
Nucleic Acids Research null
Genome-wide DNA methylation profiles in hematopoietic stem and progenitor cells reveal overrepresentation of ETS transcription factor binding sites.
Hogart, A; Lichtenberg, J; Ajay, SS; Anderson, S; , ; Margulies, EH; Bodine, DM
Genome Research null
Bairu Wang et al.
Archives of toxicology, 90(2), 449-461 (2014-12-21)
Abnormal expression of miRNAs has been implicated in the pathogenesis of human lung cancers, most of which are attributable to cigarette smoke. The mechanisms of action, however, remain obscure. Here, we report that there are decreased expression of miR-218 and
View All Related Papers

Related Content

Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

New Products: Volume 3, 2012

New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins

Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.

An Introduction to Antibodies and Their Applications

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

See All

Global Trade Item Number

SKUGTIN
17-1008504053252007262

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service