Application
Representative lot data.
Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Mouse IgG, or 4 µg of Anti-HDAC3 and the TM=["MAGNA CHIP"] A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of HDAC3 associated DNA fragments was verified by qPCR using ChIP Primers, p21 .(Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-TM=["MAGNA CHIP"] A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was probed with Anti-HDAC3 (1 µg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates HDAC3 (~49 kDa). An uncharacterized band appears at ~22 in some lysates. (Figure 3).
Disclaimer
General description
SIZE: 428 amino acids; 48848 Da
SUBUNIT: Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1 and SRY. Interacts with BTBD14B. Interacts with GLIS2 (By similarity). Interacts with CBFA2T3.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Widely expressed.
PTM: Sumoylated in vitro.
SIMILARITY: SwissProt: O15379 ## Belongs to the histone deacetylase family. Type 1 subfamily.
Other Notes
Packaging
Physical form
Concentration: 1 mg/mL
Normal Mouse IgG. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, p21. One vial containing 75 μL of each primer (5 μM) specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at
-20°C.
FOR: CCC ACA GCA GAG GAG AAA GAA
REV: CTG GAA ATC TCT GCC CAG ACA
Preparation Note
Storage Class
10 - Combustible liquids
wgk
WGK 2
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Related Content
Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 17-10238 | 04053252639678 |
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