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Merck

171536

AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, S. frugiperda

full length enzyme consisting of subunits A1, B1, and G1

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About This Item

NACRES:
NA.77
UNSPSC Code:
12352202

Product Name

AMPK (α1/β1/γ1), His•Tag®, Human, Recombinant, S. frugiperda, full length enzyme consisting of subunits A1, B1, and G1

recombinant

expressed in insect cells (S. frugiperda)

assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Disclaimer

Toxicity: Standard Handling (A)

General description

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1).
M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)

Other Notes

Scott J.W., 2007. EMBO J26, 806.
Minokoshi Y et al. 2004. Nature428, 569.

Physical form

In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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