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Merck

17-229

Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit

Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit used to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum.

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.52
eCl@ss:
32161000

Key Documents

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Product Name

Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit, Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit used to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum.

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable

NCBI accession no.

NM_003538.3

UniProt accession no.

P62805

shipped in

dry ice

Quality Level

100

Related Categories

Application

Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit used to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher using quantitative PCR or Southern slot-blot analysis, using promotor specific primers or probe.

Other Notes

Anti-acetyl-Histone H4 (Cat.# 06-866)

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Packaging

Kit capacity: 22 assays

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

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Lata Balakrishnan et al.
Journal of molecular biology, 365(1), 18-30 (2006-10-24)
SV40 chromosomes undergoing transcription operationally defined by the presence of RNA polymerase II (RNAPII) were immune-selected with antibody to RNAPII and subjected to secondary chromatin immunoprecipitation with antibodies to hyperacetylated or unacetylated H4 or H3. Immune selection fragmentation and immunoprecipitation
Shaojing Chang et al.
Journal of immunology (Baltimore, Md. : 1950), 181(12), 8372-8381 (2008-12-04)
Forming and removing epigenetic histone marks at gene loci are central processes in differentiation. Here, we explored mechanisms establishing long-range H4 acetylation marks at the Ifng locus during Th1 lineage commitment. In Th0 cells, histone deacetylase (HDAC)-Sin3A complexes recruited to
Priya Kapoor-Vazirani et al.
Cancer research, 68(16), 6810-6821 (2008-08-15)
Epigenetic silencing of tumor suppressor genes in human cancers is associated with aberrant methylation of promoter region CpG islands and local alterations in histone modifications. However, the mechanisms that drive these events remain unclear. Here, we establish an important role
Guocheng He et al.
Molecular and cellular biology, 22(9), 2965-2973 (2002-04-10)
Repression of human immunodeficiency virus type 1 (HIV-1) transcription may contribute to the establishment or maintenance of proviral quiescence in infected CD4(+) cells. The host factors YY1 and LSF cooperatively recruit histone deacetylase 1 (HDAC1) to the HIV-1 long terminal
Melker Göransson et al.
International journal of cancer, 115(4), 556-560 (2005-02-03)
The myxoid/round cell liposarcoma oncogene FUS-DDIT3 is the result of a translocation derived gene fusion between the splicing factor FUS and DDIT3. In order to investigate the downstream targets of DDIT3, and the transforming effects of the FUS-DDIT3 fusion protein
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Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

Global Trade Item Number

SKUGTIN
17-22904053252025419

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