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172426

Glucoamylase, Rhizopus sp.

Name: Glucoamylase, <i>Rhizopus sp.</i>
Brand: MM

Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. Useful for the enzymatic determination of starch.

Synonym(s):

Glucoamylase, Rhizopus sp., Glucan 1,4-α-glucosidase, Glucoamylase, Amyloglucosidase, γ-Amylase, Lysosomal α-glucosidase, exo-4,7-α-glucosidase

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About This Item

CAS Number:

9032-08-0

EC Number:

3.2.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00081350

UNSPSC Code:

12352202

NACRES:

NA.54

biological source

fungus (Rhizopus spp.)

Quality Level

100

form

lyophilized

specific activity

≥10 unit/mg solid
≥20 units/mg protein

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

solubility

aqueous buffer: 1 mg/mL
water: 1 mg/mL

shipped in

wet ice

storage temp.

−20°C

Related Categories

Glycobiology Profiling Tools

Proteins & Enzymes

General description

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Other Notes

Hiromi, K., et al. 1973. Biochim. Biophys. Acta302, 362.
Kelly, J.J. and Alpers, D.H. 1973. Biochim. Biophys. Acta315, 113.
Jeffrey, P.L., et al. 1970. Biochemistry9, 1403.
Brown, B. and Brown, D.H. 1965. Biochim. Biophys. Acta110, 124.
Tsujisaka, Y., et al. 1958. Nature181, 770.
Miller, K.D. and Copeland, W.H. 1956. Biochim. Biophys. Acta22, 193.
French, D. and Knapp, D.W. 1950. J. Biol. Chem.187, 463.

One unit is defined as the amount of enzyme that will liberate 1 µmol glucose from maltopentaose per min at 37°C, pH 6.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)