17-327

H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)

Name: H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)
Brand: MM

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About This Item

NACRES:

NA.54

UNSPSC Code:

12161503

packaging

kit of 192 assay(s)

manufacturer/tradename

Upstate^®

technique(s)

ELISA: suitable

detection method

chemiluminescent

Quality Level

100

Analysis Note

The H2A.X Phosphorylation, Chemiluminescence Detection Assay Kit is a cell-based ELISA formatted for chemiluminescent detection of relative levels of phosphorylated H2A.X in microplate cells cultures. Adherent cells are cultured in microplates, treated with agents that induce DNA damage/H2A.X phosphorylation, and are then fixed and permeabilized. Histone H2A.X phosphorylated at serine 139 is detected by Anti-phospho-H2A.X (Ser139), clone JBW301 and an anti-mouse-HRP (horseradish peroxidase) conjugate. The chemiluminescent HRP substrate LumiGLO is then added, and signal is measured in a microplate luminometer

Application

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection) is a cell based assay formatted for microplate-based detection of levels of phosphorylated histone H2A.X.

Other Notes

Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (Cat.# 05-636)
TBS, 20X (Cat.# 20-190)
20% Tween-20 (v/v)
10% BSA in TBS (Blocking Buffer)
LumiGLO Substrate Reagent A
LumiGLO Substrate Reagent B
Goat Anti-Mouse IgG, HRP conjugate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

Evading Apoptosis: Cell Based Assays

Evading Apoptosis: Cell Based Assays. As leaders in cancer research, EMD Millipore scientists have developed a wide range of assays and reagents to help uncover apoptosis mechanisms and possible ways to induce apoptosis in tumor cells for therapeutic benefit.

Global Trade Item Number

SKU	GTIN
17-327	04053252010859

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