Key Documents
176872
Angiotensin Converting Enzyme-2, His•Tag®, Human, Recombinant, NSO Cells
Synonym(s):
ACE Homolog, ACEH, ACE-2, Peptidyl-Dipetidase A, ACE
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
recombinant
expressed in NSO cells
Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥0.8 units/μg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
impurities
≤1.0 EU/μg Endotoxins (EU/μg ACE-2)
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. ACE-2 cleaves angiotensins I and II as a carboxypeptidase and plays an important role in regulating cardiovascular and renal functions. Native ACE-2 is an integral membrane zinc metalloprotease whose activity is inhibited by EDTA. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing conditions.
Packaging
Please refer to vial label for lot-specific concentration.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-YVADAPK(DNP)-OH per min at 25°C, pH 7.5.
Tikellis, C., et al. 2003. Hypertension41, 390.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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