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Merck

176904

Anthrax Lethal Factor Protease Substrate III, Fluorogenic

An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax lethal factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM.

Synonym(s):

Anthrax Lethal Factor Protease Substrate III, Fluorogenic, Anthrax LF Protease Substrate III, Fluorogenic, Bacillus anthracis LF Protease Substrate III, Fluorogenic

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About This Item

Empirical Formula (Hill Notation):
C91H156N42O18
Molecular Weight:
2126.48
UNSPSC Code:
12352200

Key Documents

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assay

≥97% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

solubility

water: 1 mg/mL, 5% acetic acid: 5 mg/mL, DMSO: 5 mg/mL

fluorescence

λex ~360 nm, λem ~460 nm

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

General description

An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax Lethal Factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors. Excitation max.: ~360 nm; emission max.: ~460 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Measuring Anthrax lethal factor (LF) metalloproteolytic activity
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Ac-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-AMC
Tonello, F., et. al. 2002. Nature418, 386.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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