178615
Anti-Aquaporin 5 Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-AQP5
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
rat, mouse, human
Application:
—
Citations:
24
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (0.5-1.0 µg/mL)
Immunoblotting (1-10 µg/mL)
Immunofluorescence (2-20 µg/mL)
Immunohistochemistry (2-20 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.
Immunogen
a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5
Application
ELISA (0.5-1.0 µg/ml)
Immunoblotting (1-10 µg/ml)
Immunofluorescence (2-20 µg/ml)
Immunohistochemistry (2-20 µg/ml)
Immunoblotting (1-10 µg/ml)
Immunofluorescence (2-20 µg/ml)
Immunohistochemistry (2-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Epithelial cells
Epithelial cells
Other Notes
Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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