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Merck

189724

Ascorbate Oxidase, Cucurbita sp.

Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

Synonym(s):

Ascorbate Oxidase, Cucurbita sp.

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About This Item

CAS Number:
9029-44-1
EC Number:
1.10.3.3 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00130560

Product Name

Ascorbate Oxidase, Cucurbita sp., Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.

form

lyophilized

specific activity

≥1500 U/mg
≥300 units/mg material

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Disclaimer

Toxicity: Standard Handling (A)
enzyme will denature below pH 4.0.

General description

Native ascorbate oxidase from Cucurbita species. A multi-copper-containing oxidase that catalyzes oxidation of ascorbic acid to dehydroascorbic acid. AO catalyses the four-electron reduction of dioxygen to water with concominant one-electron oxidation of the reducing organic substrate. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.

Other Notes

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol ascorbic acid per min at 37°C, pH 5.6.

Preparation Note

Following reconstitution refrigerate (4°C). This Stock solutions are stable for up to 3 weeks at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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