Streptavidin, Phycoerythrin Conjugate

Name: Streptavidin, Phycoerythrin Conjugate
Brand: MM

This Streptavidin, Phycoerythrin Conjugate is validated for use in Enzyme Immunoassay, Flow Cytometry.

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About This Item

NACRES:

NA.41

UNSPSC Code:

41116133

Product Name

Streptavidin, Phycoerythrin Conjugate, This Streptavidin, Phycoerythrin Conjugate is validated for use in Enzyme Immunoassay, Flow Cytometry.

biological source

(Streptomyces avidinii)

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze
protect from light

dilution

(Enzyme Immunoassay
Flow Cytometry (1-3 µg per 1 x 10 cells in 100 µL))

fluorescence

λ_ex 500 nm
λ_em 575 nm

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Application

Enzyme Immunoassay (see comments)

Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)

Disclaimer

Toxicity: Standard Handling (A)

General description

Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Other Notes

Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.
Starling, J.J., et al. 1988. Cancer Res.48, 6211.

The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 10⁶. Excitation Max: 500 nm emission max: 575 nm.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.

Preparation Note

Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c