189738
Streptavidin, TEXAS RED® Conjugate
This Streptavidin, TEXAS RED® Conjugate is validated for use in ELISA, Flow Cytometry, Immunohistochemistry.
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About This Item
NACRES:
NA.47
UNSPSC Code:
41116133
Clone:
-
Species reactivity:
-
Application:
—
Citations:
-
biological source
(Streptomyces avidinii)
Quality Level
form
liquid
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze, protect from light
dilution
(ELISA (1:2000-1:5000)
Flow Cytometry (1:50-1:200)
Immunohistochemistry (1:50-1:200))
shipped in
ambient
storage temp.
2-8°C
General description
Streptavidin conjugated to TEXAS RED® fluorescent dye. Specific for biotin, biotinylated proteins, or biotinylated antibodies.
Application
ELISA (1:2000-1:5000)
Flow Cytometry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Flow Cytometry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.
Preparation Note
Do not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.
Other Notes
Biotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 106 cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
Disclaimer
Toxicity: Standard Handling (A)
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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