196810
Bax-Inhibiting Peptide, V5
A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection.
Synonym(s):
Bax-Inhibiting Peptide, V5, BIP-V5, H-VPMLK-OH
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About This Item
Empirical Formula (Hill Notation):
C27H50N6O6S
Molecular Weight:
586.79
NACRES:
NA.77
UNSPSC Code:
12352202
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Quality level:
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Quality Level
assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
white
solubility
water: 1 mg/mL
shipped in
wet ice
storage temp.
−20°C
General description
A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).
A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Bax mediated apoptosis
Bax mediated apoptosis
Product does not compete with ATP.
Reversible: no
Target IC50: 50-200 µM against Bax mediated apoptosis
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.
Other Notes
H-Val-Pro-Met-Leu-Lys-OH
Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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