203189
Biotin-X-NHS, Water-Soluble
Synonym(s):
Biotin-X-NHS, Water-Soluble, Sulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
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About This Item
Empirical Formula (Hill Notation):
C20H29N4O9S2 · Na
Molecular Weight:
556.59
NACRES:
NA.41
UNSPSC Code:
41116133
biological source
(Synthetic chemical)
assay
≥94% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
color
off-white to tan
solubility
DMSO: 13 mg/mL
distilled water: 5.5 mg/mL
shipped in
ambient
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Readily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.
Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
Other Notes
Gretch, D.R., et al. 1987. Anal. Biochem.163, 270.
Preparation Note
Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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