203638
BMP-1/PCP, CF, Human, Recombinant
Synonym(s):
Procollagen C-proteinase, PCP
assay
≥95% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
>2 pmol/min/µg as determined by cleavage of a fluorogenic peptide substrate, Mca-YVADAPK (Dnp)-OH
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, human bone morphogenetic protein (BMP-1) consisting of amino acids 121-730 and expressed in NS0-derived mouse myeloma cells with a C-terminal His•Tag sequence.
Recombinant, human bone morphogenetic protein (BMP-1) consisting of amino acids 121-730 and expressed in NS0-derived mouse myeloma cells with a C-terminal His•Tag sequence. Will cleave a fluorogenic peptide substrate.
Other Notes
Steiglitz, B.M., et al. 2004. J. Biol. Chem.279, 980.
Hartigan, N., et al. 2003. J. Biol. Chem.278, 18045.
Leighton, M. and K.E. Kadler. 2003. J. Biol. Chem.278, 18478.
Bond, J.S. and R.J. Beynon. 1995. Protein Sci.4, 1247.
Wozney, J.M., et al. 1988. Science242, 1528.
Hartigan, N., et al. 2003. J. Biol. Chem.278, 18045.
Leighton, M. and K.E. Kadler. 2003. J. Biol. Chem.278, 18478.
Bond, J.S. and R.J. Beynon. 1995. Protein Sci.4, 1247.
Wozney, J.M., et al. 1988. Science242, 1528.
Physical form
Lyophilized from 0.2 µm filtered HEPES, NaCl, CaCl₂, ZnCl₂, and Brij®35 detergent.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are good for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile 25 mM HEPES and 0.01% Brij35, pH 7.5.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 1
Certificates of Analysis (COA)
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