203702
Brain Derived Neurotrophic Factor, Human, Recombinant, E. coli
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About This Item
UNSPSC Code:
12352202
assay
≥97% (HPLC)
Quality Segment
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, human brain drived neurotrophic factor expressed in E. coli. Promotes survival and growth of neurons in the central nervous system. Sequence analysis indicates that brain derived neurotrophic factor is structurally and functionally related to nerve growth factor. mRNA for brain derived neurotrophic factor is found predominantly in the central nervous system, whereas mRNA for nerve growth factor is also found in many non-CNS tissues.
Biochem/physiol Actions
Supports survival of rat basal forebrain primary septal cultured neurons. Induces choline acetyltransferase (EC₅₀ = 50 ng/ml)
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to a concentration of ≥10 µg/ml with sterile distilled H₂O or aqueous buffers. For more dilute solutions, add a stabilizing protein such as 0.1% BSA or HSA.
Other Notes
Erickson, J.T., et al. 1996. J. Neurosci. 16, 5361.
Leibrock, J., et al. 1989. Nature341, 149.
Leibrock, J., et al. 1989. Nature341, 149.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
Certificates of Analysis (COA)
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