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Merck

204885

Complement C3, Human

Complement C3, Human, CAS 80295-41-6, is a native C3 component. Glycoprotein composed of two non-identical disulfide-bonded subunits of 115 kDa (α) and 75 kDa (β).

Synonym(s):

Complement C3, Human

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About This Item

CAS Number:
80295-41-6
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
MFCD00130836

Product Name

Complement C3, Human, Complement C3, Human, CAS 80295-41-6, is a native C3 component. Glycoprotein composed of two non-identical disulfide-bonded subunits of 115 kDa (α) and 75 kDa (β).

assay

≥85% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

impurities

IgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin, trace

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Biochem/physiol Actions

>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.

Disclaimer

Toxicity: Standard Handling (A)

General description

Native, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115,000 (α) and 75,000 (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at the C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77, resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. The covalent attachment of C3b to target acceptors is required for continuation of complement activation.

Other Notes

Lambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol.23, 1237.
Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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