207005
Calcineurin Assay Kit, Colorimetric
Synonym(s):
Colorimetric, PP2B Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 96 tests
manufacturer/tradename
Calbiochem®
packaging
pkg of 96-well plate(s)
storage condition
OK to freeze
detection method
colorimetric
shipped in
dry ice
storage temp.
−70°C
Quality Level
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS
Features and Benefits
Assay time: 3.5 h
General description
A colorimetric assay kit for measuring calcineurin (PP2B) activity and for inhibitor screening. The assay is performed using RII Phosphopeptide Substrate (Cat. No. 207008).
Other Notes
Human Recombinant Calcineurin, Bovine Brain Calmodulin, RII Phosphopeptide Substrate, Assay Buffer, Phosphate Detection Reagent, Phosphate Calibration Standard, ½ volume 96-Well Plate, and a user protocol.
Mondragon, A. et al. 1997. Biochemistry 36, 4934.
Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.
Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
Enz, A., et al. 1994. Anal. Biochem. 216, 147.
Harder, K.W., et al. 1994. Biochem. J. 298, 395.
Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.
Packaging
1 kit in Glass bottle
Preparation Note
Store all components except the 96-Well Plate (room temperature) at -70°C for the highest stability. In particular, calcineurin must be handled with utmost care in order to retain its maximal enzymatic activity. Thaw it quickly in a water bath, maintained at room temperature, or by rubbing between fingers, then immediately store on an ice bath. Any remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, we recommend to aliquot the calcineurin into separate tubes and then store at -70°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
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