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Merck

208706

Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.

Synonym(s):

CaM Kinase II

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About This Item

UNSPSC Code:
12352202

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Product Name

Ca2+/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda, Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.

recombinant

expressed in insect cells (S. frugiperda)

assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥250,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

Phosphatases, proteases, none detected

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.
Tashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween® 20 Detergent, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

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Mirza Zizak et al.
The Journal of biological chemistry, 287(16), 13442-13456 (2012-03-01)
The epithelial brush border (BB) Na(+)/H(+) exchanger 3 (NHE3) accounts for most renal and intestinal Na(+) absorption. Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) inhibits NHE3 activity under basal conditions in intact intestine, acting in the BB, but the mechanism is unclear.

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