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208730

Sigma-Aldrich

Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3)

liquid, clone 28F3, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

28F3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, bovine

should not react with

mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

General description

Purified mouse monoclonal antibody. Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2).
Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2). Recognizes both native and denatured forms of the small subunit of calpain.
This Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Calpain-1/2, Small Subunit.

Immunogen

Human Placenta
purified calpain from human placenta

Application

ELISA (1:2000)

Immunoblotting (1:2000)

Immunoprecipitation (1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
MDBK cells

Other Notes

Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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