Key Documents

COA/COQ

217570

Anti-CD3 Mouse mAb (UCHT1)

Name: Anti-CD3 Mouse mAb (UCHT1)
Brand: MM

liquid, >95% (immunoglobulin, SDS-PAGE), clone UCHT1

Synonym(s):

Anti-CD3 Antibody, CD3 Detection Antibody, Mouse Anti-CD3

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

UCHT1, monoclonal

Assay

>95% (immunoglobulin, SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamycin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation
T cell Co-stimulation )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Anti-CD3, mouse monoclonal, clone UCHT1, recognizes the ε-chain of the CD3 complex in human peripheral T cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Protein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.

Recognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.

Immunogen

human thymocytes/Sezary T cells

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Other Notes

Thibault, G. and Bardos, P. 1995. J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol.11, 329.

This antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 10⁵ cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)