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Merck

217590

Anti-CD40 Mouse mAb (EA-5)

liquid, ≥95% (SDS-PAGE), clone EA-5

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
EA-5, monoclonal
Species reactivity:
human
Application:
Citations:

Key Documents

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Product Name

Anti-CD40 Mouse mAb (EA-5), liquid, ≥95% (SDS-PAGE), clone EA-5

form

liquid

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

EA-5, monoclonal

assay

≥95% (SDS-PAGE)

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Flow cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation
Paraffin Sections )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Application

Flow cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Paraffin Sections (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.
Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.
Recognizes the ~44 kDa CD40 protein in Raji cells.

Immunogen

transfected murine L cells expressing human CD40

Other Notes

FACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Fanslow, W.C., et al. 1992. J. Immunol.149, 655.
Law, C.L., et al. 1990. Leukemia4, 732.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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