Anti-CD40L Mouse mAb (24-31)

Name: Anti-CD40L Mouse mAb (24-31)
Brand: MM

liquid, ≥95% (SDS-PAGE), clone 24-31

Synonym(s):

Anti-gp39

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Anti-CD40L Mouse mAb (24-31), liquid, ≥95% (SDS-PAGE), clone 24-31

biological source

mouse

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

24-31, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamycin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Flow Cytometry (10 µg/mL)
Frozen Sections
Immunoprecipitation
Neutralization Studies
Immunoblotting )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Application

Flow Cytometry (10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Immunoblotting (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CD40L, mouse monoclonal, clone 24-32, recognizes the ~36 kDa CD40L (gp39) on activated CD3+ human PBL. It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Note: 1 T = 1 test.

Protein A affinity purified mouse monoclonal antibody. Recognizes the ~39-40 kDa CD40L protein.

Recognizes the ~36 kDa CD40L (gp39) protein on activated CD3+ human PBL.

Immunogen

Human

human sgp39 fusion protein

Other Notes

This antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x10⁵ ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c