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Merck

217605

Anti-CD54 Mouse mAb (15.2)

liquid, ≥95% (SDS-PAGE), clone 15.2

Synonym(s):

Anti-ICAM-1, Domain 1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
15.2, monoclonal
Species reactivity:
human
Application:
Citations:

Key Documents

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Product Name

Anti-CD54 Mouse mAb (15.2), liquid, ≥95% (SDS-PAGE), clone 15.2

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

15.2, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamicin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Flow Cytometry (1-10 µg/mL)
Frozen Sections
Immunoblotting
Immunoprecipitation
Neutralization Studies )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Positive Control
Raji cells

Application

Flow Cytometry (1-10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.
Protein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.

Immunogen

Human
human monocytes

Other Notes

FACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell68, 71.
Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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