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Key Documents

COA/COQ

217605

Anti-CD54 Mouse mAb (15.2)

Name: Anti-CD54 Mouse mAb (15.2)
Brand: MM

liquid, ≥95% (SDS-PAGE), clone 15.2

Synonym(s):

Anti-ICAM-1, Domain 1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

15.2, monoclonal

Assay

≥95% (SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamicin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Flow Cytometry (1-10 µg/mL)
Frozen Sections
Immunoblotting
Immunoprecipitation
Neutralization Studies )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Protein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).

Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.

Immunogen

Human

human monocytes

Application

Flow Cytometry (1-10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Analysis Note

Positive Control
Raji cells

Other Notes

FACS was performed on 5x10⁵ cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell68, 71.
Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)