217605
Anti-CD54 Mouse mAb (15.2)
liquid, ≥95% (SDS-PAGE), clone 15.2
Synonym(s):
Anti-ICAM-1, Domain 1
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
15.2, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
15.2, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
0.5 mg/mL gentamicin sulfate as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (1-10 µg/mL)
Frozen Sections
Immunoblotting
Immunoprecipitation
Neutralization Studies )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.
Protein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
Immunogen
Human
human monocytes
Application
Flow Cytometry (1-10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Physical form
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Positive Control
Raji cells
Raji cells
Other Notes
FACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell68, 71.
Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.
Berendt, A.R., et al. 1992. Cell68, 71.
Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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