Skip to Content
Merck

217620

Anti-CD64 Mouse mAb (10.1)

liquid, ≥95% (SDS-PAGE), clone 10.1

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
10.1, monoclonal
Species reactivity:
human
Application:
Citations:

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-CD64 Mouse mAb (10.1), liquid, ≥95% (SDS-PAGE), clone 10.1

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

10.1, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

0.5 mg/mL gentamycin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Flow Cytometry (10 µg/mL)
Frozen Sections
Immunoblotting
Immunoprecipitation
Neutralization Studies )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Negative Control
HPB-MLT
Positive Control
4937 cells

Application

Flow Cytometry (10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.
Recognizes the ~72 kDa CD64 protein (FcR1 receptor).

Immunogen

Human
human rheumatoid synovial fluid cells/monocytes

Other Notes

Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.
The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service