217660
Anti-CD18 Mouse mAb (IB4)
liquid, ≥95% (SDS-PAGE), clone IB4
Synonym(s):
Anti-β2 Integrin
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
IB4, monoclonal
Species reactivity:
human
Application:
—
Citations:
4
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
IB4, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
0.5 mg/mL gentamicin sulfate as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (5-10 µg/mL)
Frozen Sections
Immunoprecipitation
Cell Inhibition Assays )
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
Immunogen
Human
human peripheral blood monocytes
Application
Flow Cytometry (5-10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Negative Control
Nalm-6 cells
Nalm-6 cells
Positive Control
HPB-MLT cells
HPB-MLT cells
Other Notes
Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.
Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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