218712
Anti-Casein Kinase II, β-Subunit Mouse mAb (6D5)
lyophilized, clone 6D5, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
6D5, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human, mouse, hamster, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
Anti-Casein Kinase II, β-Subunit, mouse monoclonal, clone 6D5, recognizes recombinant CKII β-subunit and native holoenzyme. It is validated for Western blotting and immunocytochemistry.
Protein A purified mouse monoclonal antibody. Recognizes the ~25 kDa CKII β-subunit.
Recognizes recombinant CKII β-subunit and native holoenzyme.
Immunogen
Human
purified, recombinant, human CKII β-subunit
Application
Immunoblotting (5 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunocytochemistry (5 µg/ml)
Physical form
Lyophilized from PBS.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Other Notes
Nastainczyk, W., et al. 1995. Hybridoma 14, 335.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B
Storage Class
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Aron Szabó et al.
PLoS biology, 11(8), e1001645-e1001645 (2013-09-10)
Phosphorylation is a pivotal regulatory mechanism for protein stability and activity in circadian clocks regardless of their evolutionary origin. It determines the speed and strength of molecular oscillations by acting on transcriptional activators and their repressors, which form negative feedback
Janeen H Trembley et al.
Molecular and cellular biochemistry, 356(1-2), 21-35 (2011-07-16)
CK2 is a highly conserved, ubiquitous, signal responsive protein serine/threonine kinase. CK2 promotes cell proliferation and suppresses apoptosis, and increased CK2 expression is observed in all cancers examined. We previously reported that direct injection of antisense (AS) CK2α phosphorothioate oligonucleotides
D Vidal et al.
Plant biology (Stuttgart, Germany), 12(1), 134-144 (2010-07-27)
Two calcium- and light-dependent protein kinases have been reported in etiolated Cucumis sativus cotyledons (Vidal et al. 2007). In the present work, we studied casein kinase (CK) activity in etiolated cucumber cotyledons of in-gel and in vitro kinase assays, using