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218765

Caspase-9 Substrate I, Fluorogenic

Name: Caspase-9 Substrate I, Fluorogenic
Brand: MM

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

Synonym(s):

Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC

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About This Item

Empirical Formula (Hill Notation):

C₃₃H₃₈F₃N₇O₁₁

CAS Number:

210345-03-2

Molecular Weight:

765.69

MDL number:

MFCD01862610

UNSPSC Code:

12352200

NACRES:

NA.77

Assay:

≥95% (HPLC)

Form:

solid

Storage condition:

OK to freeze, desiccated, protect from light

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Properties

Quality Segment

100

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated, protect from light

color

white

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex ~400 nm, λ_em ~505 nm

shipped in

ambient

storage temp.

−20°C

SMILES string

FC(F)(F)C1=CC(=O)Oc2c1ccc(c2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(C)C)CCC(=O)O)Cc3[nH]cnc3)CC(=O)O

InChI

1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)

InChI key

HULKIXRFKRCRHD-ZJZGAYNASA-N

Description

General description

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A fluorogenic substrate for caspase-9

Product does not compete with ATP.

Reversible: no

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ac-Leu-Glu-His-Asp-AFC

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c