218813
Caspase-2, Human, Recombinant, E. coli
Caspase-2, Human, Recombinant, E. coli is expressed as a single polypeptide chain that undergoes spontaneous autoprocessing to yield the native active enzyme.
Synonym(s):
ICH-1, Nedd-2
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About This Item
UNSPSC Code:
12352202
Specific activity:
≥1500 units/mg protein
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
lyophilized solid
specific activity
≥1500 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
PBS: 0.1-1 units/μL
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Recombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Other Notes
One unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.
Talanian, R.V., et al. 1997. J. Biol. Chem.272, 9677.
Wang, L., et al. 1994. Cell78, 739.
Wang, L., et al. 1994. Cell78, 739.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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