Caspase-3 Substrate IX, Fluorogenic

Name: Caspase-3 Substrate IX, Fluorogenic
Brand: MM

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate.

Synonym(s):

Caspase-3 Substrate IX, Fluorogenic, (Z-DEVD)_2-Rh 110

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About This Item

Empirical Formula (Hill Notation):

C₇₂H₇₈N₁₀O₂₇

Molecular Weight:

1515.44

UNSPSC Code:

12352200

Product Name

Caspase-3 Substrate IX, Fluorogenic, A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate.

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

DMSO: 1 mg/mL

fluorescence

λ_ex ~485 nm
λ_em ~535 nm

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Cell permeable: no

Primary Target
A highly sensitive, photostable caspase-3 fluorogenic substrate

Product does not compete with ATP.

Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.

Other Notes

(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110

Hug, H., et al. 1999. Biochemistry38, 13906.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c