Anti-β-Catenin, Dephospho Mouse mAb (8E4)

Positive Control
LiCl-treated SW480 cells (included)

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)

Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)

Toxicity: Standard Handling (A)

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x10⁶ LiCl-treated SW480 cells.

Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x10⁶ LiCl-treated SW480 cells.

This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.

Mouse

a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH

SW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.

100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.

Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

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