234376
Macrophage Colony-Stimulating Factor, Human, Recombinant, E. coli
Synonym(s):
Macrophage Colony-Stimulating Factor, Human, Recombinant, E. coli, rhM-CSF
assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg Endotoxin (EU/μg M-CSF)
shipped in
dry ice
storage temp.
−70°C
Quality Level
General description
Recombinant, human macrophage colony-stimulating factor expressed in E. coli. Stimulates formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes. Human M-CSF is active in murine systems and the recombinant cytokine is indistinguishable from the natural M-CSF in all biological assays tested.
Biochem/physiol Actions
ED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
Physical form
Lyophilized from sterile-filtered PBS, 50 µg of BSA/µg M-CSF.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
Reconstitute to a concentration of ≥1 mg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Other Notes
Edwards, M., et al. 1998. Bone 22, 325.
Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun.177, 526.
Bergamini, A., et al. 1994. Immunol. Lett. 42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys. Res. Commun.177, 526.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 2
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