234378
Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli
Synonym(s):
rmM-CSF
assay
≥97% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotoxin (EU/ug M-CSF)
shipped in
ambient
storage temp.
−70°C
Quality Level
Biochem/physiol Actions
ED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, mouse macrophage colony-stimulating factor expressed in E. coli. Stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes.
Stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes.
Other Notes
Bergamini, A., et al. 1994. Immunol. Lett.42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys.Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA85, 6706.
Hattersley, G., et al. 1991. Biochem. Biophys.Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA85, 6706.
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg M-CSF.
Preparation Note
Following reconstitution, aliquot and freeze(-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
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G Hattersley et al.
Biochemical and biophysical research communications, 177(1), 526-531 (1991-05-31)
The op/op mouse, in which the M-CSF gene is mutated, has greatly reduced numbers of macrophages and osteoclasts. We assessed the ability of M-CSF to induce osteoclast and macrophage formation in op/op hemopoietic cells in vitro. Osteoclast production was undetectable
A Bergamini et al.
Immunology letters, 42(1-2), 35-40 (1994-09-01)
We show here that HIV-infected monocyte-macrophages stimulated by macrophage-colony stimulating factor (M-CSF) undergo massive syncytia formation and die. The M-CSF-stimulated HIV-infected monocyte-macrophages (M/M) destroy themselves by blebbing out particles (resembling apoptotic bodies) which may contain condensed and marginated chromatin. The
M B Ladner et al.
Proceedings of the National Academy of Sciences of the United States of America, 85(18), 6706-6710 (1988-09-01)
A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino
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