Key Documents
234378
Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli
Synonym(s):
rmM-CSF
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About This Item
UNSPSC Code:
12352202
Assay
≥97% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotoxin (EU/ug M-CSF)
shipped in
ambient
storage temp.
−70°C
General description
Recombinant, mouse macrophage colony-stimulating factor expressed in E. coli. Stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes.
Stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. Induces cell death in HIV-1 infected monocytes.
Biochem/physiol Actions
ED₅₀ = 0.5-1.5 ng/ml as measured in a cell proliferation assay using the M-CSF-dependent mouse monocytic cell line, M-NFS-60
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg M-CSF.
Preparation Note
Following reconstitution, aliquot and freeze(-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Other Notes
Bergamini, A., et al. 1994. Immunol. Lett.42, 35.
Hattersley, G., et al. 1991. Biochem. Biophys.Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA85, 6706.
Hattersley, G., et al. 1991. Biochem. Biophys.Res. Commun. 177, 526.
Ladner, M.B., et al. 1988. Proc. Natl. Acad. Sci. USA85, 6706.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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