235417
Caspase-3, Human, Recombinant, E. coli
Caspase-3, Human, Recombinant, is expressed as a proenzyme in E. coli and is subsequently cleaved to produce the active enzyme.
Synonym(s):
YAMA, Apopain, CPP32, YAMA, CPP32, Apopain
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About This Item
UNSPSC Code:
12352202
Specific activity:
≥1450 units/μg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥1450 units/μg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
M.W. 12,000 and 17,000 (heterodimer).
Recombinant, human caspase-3 expressed in E. coli as the proenzyme, which is subsequently cleaved to produce the active enzyme. The active enzyme is a heterodimer with subunit molecular weights of 12 kDa and 19 kDa. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage and inhibitor screening. Caspase-3 is a member of the ICE family of cysteine proteases that is activated during apoptotic signaling events by upstream proteases, including caspase-6, caspase-8 (FLICE) and cytotoxic T-cell-derived granzyme B.
Physical form
In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Izban, K.F., et al. 1999. Am. J. Pathol. 154, 1439.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Mittl, P.R.E., et al. 1997. J. Biol. Chem. 272, 6539.
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Mittl, P.R.E., et al. 1997. J. Biol. Chem. 272, 6539.
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
One unit is defined as the amount of enzyme that will cleave 1.0 pmol of the substrate Ac-DEVD-pNA (Cat. No. 235400) per min at 30°C, pH 7.4.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10 - Combustible liquids
wgk
WGK 2
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