Caspase-3 Substrate II, Fluorogenic

Name: Caspase-3 Substrate II, Fluorogenic
Brand: MM

Fluorogenic caspase-3 substrate.

Synonym(s):

Caspase-3 Substrate II, Fluorogenic, Ac-DEVD-AMC

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About This Item

Empirical Formula (Hill Notation):

C₃₀H₃₇N₅O₁₃

CAS Number:

169332-61-0

Molecular Weight:

675.64

UNSPSC Code:

12352200

NACRES:

NA.77

MDL number:

MFCD00671411

Product Name

Caspase-3 Substrate II, Fluorogenic, Fluorogenic caspase-3 substrate.

SMILES string

N([C@@H](C(C)C)C(=O)N[C@@H](CC(=O)O)C(=O)Nc1cc2[o][c](cc(c2cc1)C)=O)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(=O)O)CCC(=O)O

InChI

1S/C30H37N5O13/c1-13(2)26(35-27(44)18(7-8-22(37)38)33-29(46)19(11-23(39)40)31-15(4)36)30(47)34-20(12-24(41)42)28(45)32-16-5-6-17-14(3)9-25(43)48-21(17)10-16/h5-6,9-10,13,18-20,26H,7-8,11-12H2,1-4H3,(H,31,36)(H,32,45)(H,33,46)(H,34,47)(H,35,44)(H,37,38)(H,39,40)(H,41,42)/t18-,19-,20-,26-/m0/s1

InChI key

ALZSTTDFHZHSCA-RNVDEAKXSA-N

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
protect from light

color

off-white

solubility

DMSO: 10 mM

fluorescence

λ_ex 365-380 nm
λ_em 430-460 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Bioactive Small Molecule Inhibitors

Biochem/physiol Actions

Cell permeable: no

Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3

Primary Target
Fluorogenic caspase-3 substrate

Product does not compete with ATP.

Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.

Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Other Notes

Ac-Asp-Glu-Val-Asp-AMC

Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c