239764
Cycloheximide, High Purity
Antifungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes.
Synonym(s):
Cycloheximide, High Purity
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About This Item
Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
Molecular Weight:
281.35
UNSPSC Code:
12352200
MDL number:
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key
YPHMISFOHDHNIV-FSZOTQKASA-N
assay
≥98% (HPLC)
form
solid
potency
3.4 μM Ki
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
chloroform: soluble, ethanol: soluble, methanol: soluble
shipped in
ambient
storage temp.
10-30°C
Quality Level
General description
Antifungal antibiotic that does not inhibit several species of pathogenic bacteria at 100 µg/ml. Inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFkBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt′s lymphoma cells, and a variety of other cell types, including rodent macrophages. Inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.
Antifungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (Ki = 3.4 µM). Triggers apoptosis in HL-60 cells, T cell hybridomas, Burkitt’s lymphoma cells, and a variety of other cell types including rodent macrophages. However, it inhibits DNA cleavage in rat thymocytes treated with Thapsigargin (Cat. No. 586005 ), methylprednisolone, and Ionomycin (Cat. Nos. 407950 and 407952 ). Rapidly destroyed in alkaline solutions. Also available as a 300 mM solution in DMSO (Cat. No. 508739 ).
Biochem/physiol Actions
Primary Target
hFKBP12
hFKBP12
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C, pH 3-5.
Other Notes
Christner, C., et al. 1999. J. Med. Chem. 42, 3615.
Lu, Q., et al. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Cotter, T.G., et al. 1992. Anticancer Res.12, 773.
Takano, Y.S., et al. 1991. J. Pathol.163, 329.
Waring, P. 1990. J. Biol. Chem. 265, 14476.
Lu, Q., et al. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Cotter, T.G., et al. 1992. Anticancer Res.12, 773.
Takano, Y.S., et al. 1991. J. Pathol.163, 329.
Waring, P. 1990. J. Biol. Chem. 265, 14476.
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Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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