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Key Documents

25-006

Sigma-Aldrich

p53 Activating Ligand

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405

biological source

human

description

Peptide corresponding to the cell membrane-translocating sequence linked to the single-stranded DNA binding region of human p53, ′peptide 46′, corresponding to amino acids 361-382.

manufacturer/tradename

Upstate®

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

MTS Control Peptide (25-003) can be used as a control for experiments with this peptide.

Application

Peptide corresponding to the cell membrane-translocating sequence linked to the single-stranded DNA binding region of human p53, ′peptide 46′, corresponding to amino acids 361-382 (AAVALLPAVLLALLAP-GSRAHSSHLKSKKGQSTSRHKK). MW = 3932Da. The amount of peptide to be used must be empirically determined.

Physical form

lyophilized

Preparation Note

2 years at -20°C

Analysis Note

A related p53 activator peptide containing the peptide 46 sequence has been used to induce apoptosis in Ew36, BL41 and SW480 cells1,2. The amount of peptide to be used must be empirically determined.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Restoration of the growth suppression function of mutant p53 by a synthetic peptide derived from the p53 C-terminal domain.
Selivanova, G, et al.
Nature Medicine, 3, 632-638 (1997)
A Marchese et al.
The Journal of antimicrobial chemotherapy, 35(1), 53-66 (1995-01-01)
Cefdinir is one of the few oral third generation cephalosporins that shows useful activity against nosocomial Gram-positive pathogens. For this reason the anti-staphylococcal potency of the new drug, alone or in combination with other drugs was further characterized. Against penicillin-resistant
The single-stranded DNA end binding site of p53 coincides with the C-terminal regulatory region.
Selivanova, G, et al.
Nucleic Acids Research, 24, 3560-3567 (1996)

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