251760
DAR-4M
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.
Synonym(s):
DAR-4M, Diaminorhodamine-4M
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About This Item
Empirical Formula (Hill Notation):
C25H26N4O3
Molecular Weight:
430.50
NACRES:
NA.25
UNSPSC Code:
12352202
assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
red-violet
fluorescence
λex ~560 nm, λem ~575 nm
shipped in
ambient
storage temp.
−20°C
Quality Level
Related Categories
General description
A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500 ).
Biochem/physiol Actions
Cell permeable: no
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Product does not compete with ATP.
Reversible: no
Physical form
In DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
Other Notes
Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.
Legal Information
Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
Certificates of Analysis (COA)
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