DNase I, Bovine Pancreas

Name: DNase I, Bovine Pancreas
Brand: MM

Native DNase I from bovine pancreas.

Synonym(s):

DNase I, Bovine Pancreas, Deoxyribonuclease I

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About This Item

CAS Number:

9003-98-9

EC Number:

3.1.21.1 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

MDL number:

MFCD00130918

Product Name

DNase I, Bovine Pancreas, Native DNase I from bovine pancreas.

biological source

bovine pancreas

description

Merck USA index - 14, 2906

form

lyophilized

specific activity

≥60,000 dornase units/mg dry wt

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
desiccated

solubility

sodium acetate buffer: soluble
water: soluble

shipped in

ambient

storage temp.

−20°C

Disclaimer

Toxicity: Standard Handling (A)

General description

Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units

Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.

Other Notes

One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.

Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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