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DM-NITROPHEN Reagent, Tetrasodium Salt

Name: DM-NITROPHEN Reagent, Tetrasodium Salt
Brand: MM

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis.

Synonym(s):

DM-NITROPHEN Reagent, Tetrasodium Salt, 1-(2-Nitro-4,5-dimethoxyphenyl)-1,2-diaminoethane-N,N,Nʹ,Nʹ-tetraacetic Acid, 4Na, Dimethoxynitrophenamine, 4Na

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About This Item

Empirical Formula (Hill Notation):

C₁₈H₁₉N₃O₁₂ · 4Na

Molecular Weight:

561.32

NACRES:

NA.25

UNSPSC Code:

12352200

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Properties

Quality Segment

100

assay

≥93% (HPLC)

form

solid

reaction suitability

reagent type: chelator

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

orange

solubility

water: 100 mg/mL

shipped in

wet ice

storage temp.

−20°C

Description

General description

Caged Ca²⁺ chelator that undergoes a major and rapid decrease in Ca²⁺-binding affinity upon photolysis. Can be microinjected or used with permeabilized cells. May also be useful as caged Mg²⁺ chelator. K_d (Ca²⁺) = 5 nM; K_d(Mg²⁺) = 2-5 µM.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Mulkey, R.M., and Zucker, R.S. 1993. J. Physiol. 462, 243.
Zucker, R.S. 1993. Cell Calcium 14, 87.
McCray, J.A., et al. 1992. Biochemistry31, 8856.
Delaney, K.R., and Zucker, R.S. 1990. J. Physiol.426, 473.
Kaplan, J.H., and Ellis-Davies, G.C. 1988. Proc. Natl. Acad. Sci. USA85, 6571.
Morad, M., et al. 1988. Science241, 842.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c