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Merck

322123

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

Sulfhydryl reagent used to characterize reactive SH groups.

Synonym(s):

5,5′-Dithio-bis-(2-nitrobenzoic Acid), DTNB, Ellman’s Reagent

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About This Item

Empirical Formula (Hill Notation):
C14H8N2O8S2
CAS Number:
69-78-3
Molecular Weight:
396.35
UNSPSC Code:
12352200
NACRES:
NA.21
MDL number:
MFCD00007140

Product Name

5,5′-Dithio-bis-(2-nitrobenzoic Acid), Sulfhydryl reagent used to characterize reactive SH groups.

SMILES string

S(Sc2cc(c(cc2)[N+](=O)[O-])C(=O)O)c1cc(c(cc1)[N+](=O)[O-])C(=O)O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

description

RTECS - DG9650000

assay

≥98% (titration)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

ethanol: 8 mg/mL

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
Useful in photometric determination of thiols
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.

Other Notes

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

Packaging

Packaged under inert gas

Preparation Note

May require gentle heating for complete solubilization.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

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