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324717

Endoglycosidase H

Cleaves high-mannose N-glycans, suitable for Mass Spectrometry, from Streptomyces plicatus, recombinant, expressed in E. coli

Synonym(s):

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endo-β-N-acetylglucosaminidase H, Endo H

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About This Item

CAS Number:
52769-51-4
EC Number:
3.2.1.96 (BRENDA, IUBMB)
MDL number:
MFCD03791122
NACRES:
NA.54
UNSPSC Code:
12352202
Specific activity:
≥3 units/mL, ≥30 units/mg protein
Recombinant:
expressed in E. coli
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Product Name

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

recombinant

expressed in E. coli

Quality Segment

100

conjugate

(N-linked)

form

liquid

specific activity

≥3 units/mL, ≥30 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Note: 1 mU = 1 milliunit.
Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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