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324865

Sigma-Aldrich

PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:1000))

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

phosphorylation (pTyr845)

Gene Information

human ... EGFR(1956)

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr845.
Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR

Application

Immunoblotting (1:1000)

Physical form

In Dulbecco′s PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
A431 cells stimulated with EGF

Other Notes

Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem.274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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