324867
PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, mouse, chicken, rat
Application:
—
Citations:
2
biological source
rabbit
Quality Segment
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, chicken, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:1000))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr1068)
Gene Information
chicken ... Egfr(396494)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)
General description
Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1068.
Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
Application
Immunoblotting (1:1000)
Physical form
In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
Other Notes
Jo, M., et al. 2000. J. Biol. Chem.275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.
When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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