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324867

PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

Name: PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

human, mouse, chicken, rat

Application:

—

Citations:

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Properties

biological source

rabbit

Quality Segment

100

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, chicken, rat

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:1000))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr1068)

Gene Information

chicken ... Egfr(396494)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

Description

General description

Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸.

Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

This PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR

Application

Immunoblotting (1:1000)

Physical form

In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.

Other Notes

Jo, M., et al. 2000. J. Biol. Chem.275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.

When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c