341597
FGF-II, Mouse, Recombinant, E. coli
Synonym(s):
mFGF-II, Fibroblast Growth Factor-II
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About This Item
UNSPSC Code:
12352202
assay
≥95% (SDS-PAGE)
Quality Level
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.1 ng/μg Endotoxins (ng/μg FGF-2)
color
white
shipped in
wet ice
storage temp.
−20°C
General description
Full-length, recombinant, mouse basic fibroblast growth factor expressed in E. coli. A multi-functional factor with chemotactic activity for numerous cell types. It plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells. Mouse FGFII shares 95% identity with human FGFII. Useful for studies of cell activation and signaling.
Biochem/physiol Actions
ED₅₀: ≤0.5 ng/ml as determined by the dose-dependent proliferation of BALB/3T3 cells.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to 100 µg/ml with sterile H₂O.
Other Notes
Coulier, F. et al. 1997. J. Mol Evol.44, 43.
Claus, P. et al., 2003. J. Biol. Chem.278, 749.
Claus, P. et al., 2003. J. Biol. Chem.278, 749.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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