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Merck

343209

Fluorescein Diacetate

≥99% (HPLC), solid, esterase substrate, Calbiochem®

Synonym(s):

Fluorescein Diacetate

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About This Item

Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
Molecular Weight:
416.38
UNSPSC Code:
12171500
NACRES:
NA.77
MDL number:

Product Name

Fluorescein Diacetate, Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.

SMILES string

O1C3(c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C)c2c(cccc2)C1=O

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

colorless to yellow

solubility

ethanol: soluble

shipped in

ambient

storage temp.

2-8°C

Quality Level

Related Categories

Disclaimer

Toxicity: Standard Handling (A)

General description

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
Substrate for lipases and esterases. Abs. Max: 498 nm.

Other Notes

Clements, K.M., et al. 2001. Osteoarthritis Cartilage9, 499.
Bedner, E., et al. 1998. Cytometry33, 1.
Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.

Preparation Note

Unstable in solution. Reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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