343222
Biotin, Fluorescein Conjugate
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About This Item
NACRES:
NA.41
UNSPSC Code:
41116133
biological source
(Synthetic chemical)
Quality Segment
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
dilution
(EIA (1:1,000)
Tissue Staining (1:50 - 1:100))
color
orange
solubility
DMF: 5 mg/mL, DMSO: 5 mg/mL, methanol: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
It can be used for the detection of avidin and streptavidin.
Application
EIA (1:1,000)
Tissue Staining (1:50 - 1:100)
Tissue Staining (1:50 - 1:100)
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Other Notes
Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203
Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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